Analysis Note

Two distinct bands by SDS-PAGE

General description

Recombinant, human calcineurin Aα (catalytic subunit) and calcineurin B (regulatory subunit) co-expressed with yeast myristoyl-CoA:protein N-myristoyltransferase. A Ca²⁺/CaM-dependent protein phosphatase. The resulting highly active calcineurin is N-myristoylated on the CaN B-subunit, as is the native protein. M.W. 60,000 (A subunit) and 15,000 (B subunit).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Guerini, D. 1997. Biochem. Biophys. Res. Commun. 235, 271.Mondragon, A., et al. 1997. Biochemistry 36, 4934.Wei, Q. and Lee, E.Y. 1997. Biochemistry36, 7418.

Packaging

5000 u in Plastic ampoule

Physical form

In 100 mM NaCl, 50 mM Tris, 6 mM MgCl₂, 5 mM DTT, 0.5 mM CaCl₂, 0.025% NP-40.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Please refer to vial label for lot-specific reconstitution volume.

Unit Definition

One unit is defined as the amount of enzyme that will release 1.0 pmol of phosphate from the RII phosphopeptide substrate (DLDVPIPGRFDRRVpSVAAE; catNO=207008">Cat. No. 207008) in the presence of calmodulin per min at 30°C, pH 7.4.

Warning

Toxicity: Standard Handling (A)